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Dainippon Sumitomo block ace reagent
Block Ace Reagent, supplied by Dainippon Sumitomo, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/block+ace+reagent/block+ace/pm38331306-97-11-14
Average 90 stars, based on 1 article reviews
block ace reagent - by Bioz Stars, 2026-11
90/100 stars

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Binding Assay:

Article Title: Evaluation of Efficacy of Radioimmunotherapy with 90 Y-Labeled Fully Human Anti-Transferrin Receptor Monoclonal Antibody in Pancreatic Cancer Mouse Models
Article Snippet: .. 10 Materials and methods for Figure S3 11 Cells were grown on glass coverslips overnight and fixed in cold methanol for 5 min. 12 Nonspecific binding of antibodies was blocked by applying a Block Ace reagent (Dainippon 13 Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Article Title: Evaluation of Efficacy of Radioimmunotherapy with 90 Y-Labeled Fully Human Anti-Transferrin Receptor Monoclonal Antibody in Pancreatic Cancer Mouse Models
Article Snippet: AsPC-1 and BxPC-3 cells were maintained in RPMI 1640 medium (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 10% fetal bovine serum. .. Cells were grown on glass coverslips overnight and fixed in cold methanol for 5 min. Nonspecific binding of antibodies was blocked by applying a Block Ace reagent (Dainippon Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Article Title: Anti‑tissue factor antibody‑mediated immuno‑SPECT imaging of tissue factor expression in mouse models of pancreatic cancer.
Article Snippet: .. Briefly, cells were grown on glass coverslips and fixed in cold methanol for 5 min. Non‐specific binding was blocked by applying Block Ace reagent (Dainippon Pharmaceutical Co., Ltd., Osaka, Japan) with 10% goat serum for 30 min at room temperature. ..

Article Title: Preclinical evaluation of 89Zr-labeled human antitransferrin receptor monoclonal antibody as a PET probe using a pancreatic cancer mouse model
Article Snippet: Objective Pancreatic cancer is aggressive and its prognosis remains poor; thus, effective therapy is urgently needed.. Transferrin receptor (TfR) is highly expressed in pancreatic cancer and is considered to be a good candidate for molecular-targeted therapy.. We radiolabeled and evaluated fully human anti-TfR monoclonal antibodies as a new PET probe for evaluating the biodistribution of the anti-TfR antibody in pancreatic cancer.

Article Title: C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model.
Article Snippet: Introduction: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor arising from the gastrointestinal tract and highly expresses mutated c-kit.. We aimed to develop a specific and sensitive method for detecting GISTs using radiolabeled anti-c-kit monoclonal antibody.. Methods: A mutated c-kit-expressing cell clone was established by transfecting an expressing vector of mutated c-kit gene into HEK293 human embryonic kidney cells.

Blocking Assay:

Article Title: Evaluation of Efficacy of Radioimmunotherapy with 90 Y-Labeled Fully Human Anti-Transferrin Receptor Monoclonal Antibody in Pancreatic Cancer Mouse Models
Article Snippet: .. 10 Materials and methods for Figure S3 11 Cells were grown on glass coverslips overnight and fixed in cold methanol for 5 min. 12 Nonspecific binding of antibodies was blocked by applying a Block Ace reagent (Dainippon 13 Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Article Title: Evaluation of 89 Zr-Labeled Human Anti-CD147 Monoclonal Antibody as a Positron Emission Tomography Probe in a Mouse Model of Pancreatic Cancer
Article Snippet: The intensity of each band was quantified using ImageJ software (National Institute of Mental Health, Bethesda, MD, USA). .. For immunofluorescence staining, cells were grown on glass coverslips and fixed in cold methanol for 5 min. Nonspecific binding of the antibody was blocked by applying Block Ace reagent (Dainippon Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Article Title: Evaluation of Efficacy of Radioimmunotherapy with 90 Y-Labeled Fully Human Anti-Transferrin Receptor Monoclonal Antibody in Pancreatic Cancer Mouse Models
Article Snippet: AsPC-1 and BxPC-3 cells were maintained in RPMI 1640 medium (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 10% fetal bovine serum. .. Cells were grown on glass coverslips overnight and fixed in cold methanol for 5 min. Nonspecific binding of antibodies was blocked by applying a Block Ace reagent (Dainippon Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Article Title: Anti‑tissue factor antibody‑mediated immuno‑SPECT imaging of tissue factor expression in mouse models of pancreatic cancer.
Article Snippet: .. Briefly, cells were grown on glass coverslips and fixed in cold methanol for 5 min. Non‐specific binding was blocked by applying Block Ace reagent (Dainippon Pharmaceutical Co., Ltd., Osaka, Japan) with 10% goat serum for 30 min at room temperature. ..

Article Title: Preclinical evaluation of 89Zr-labeled human antitransferrin receptor monoclonal antibody as a PET probe using a pancreatic cancer mouse model
Article Snippet: Objective Pancreatic cancer is aggressive and its prognosis remains poor; thus, effective therapy is urgently needed.. Transferrin receptor (TfR) is highly expressed in pancreatic cancer and is considered to be a good candidate for molecular-targeted therapy.. We radiolabeled and evaluated fully human anti-TfR monoclonal antibodies as a new PET probe for evaluating the biodistribution of the anti-TfR antibody in pancreatic cancer.

Article Title: C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model.
Article Snippet: Introduction: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor arising from the gastrointestinal tract and highly expresses mutated c-kit.. We aimed to develop a specific and sensitive method for detecting GISTs using radiolabeled anti-c-kit monoclonal antibody.. Methods: A mutated c-kit-expressing cell clone was established by transfecting an expressing vector of mutated c-kit gene into HEK293 human embryonic kidney cells.

Article Title: Growth-related skeletal changes and alterations in phosphate metabolism.
Article Snippet: Serum inorganic phosphate (Pi) levels are higher in children than in adults; however, the underlying mechanisms remain unclear.. Therefore, we herein attempted to elucidate the mechanisms altering Pi metabolism from youth to adulthood using 4-week-old (young) and 12-week-old (adult) mice.. Despite higher serum Pi levels, serum fibroblast growth factor 23 (FGF23) levels were lower in young mice, and the amount of FGF23 in bone tended to increase from youth to adulthood.

Article Title: Complex intrinsic abnormalities in osteoblast lineage cells of X-linked hypophosphatemia: Analysis of human iPS cell models generated by CRISPR/Cas9-mediated gene ablation.
Article Snippet: Equal amounts of protein were separated on SDS-PAGE and were transferred to PVDF membranes (BioRad Laboratories, Hercules, CA). .. After blocking with Blocking One P reagent (Nacalai Tesque Inc.) or Block Ace reagent (Dainippon Pharmaceuticals, Osaka, Japan), the membranes were incubated at 4 ◦C overnight with the following primary antibodies: antiDMP1 rabbit polyclonal antibody (TaKaRa, Shiga, Japan), anti-OPN rabbit polyclonal antibody (ProteinTech, Rosemont, IL, USA), antiPiT1 (SLC20A1) rabbit polyclonal antibody (H-130; Santa Cruz Biotechnology, Santa Cruz, CA), anti-SLC20A2 (PiT-2) rabbit polyclonal antibody (ProteinTech), anti-GAPDH goat polyclonal antibody (V-18; Santa Cruz Biotechnology), anti-phosphorylated ERK1/2 antibody, antiERK1/2 antibody, anti-phosphorylated CREB (Ser133) antibody, antiCREB antibody, anti-phosphorylated FRS2α antibody (Cell Signaling Technology, Beverly, MA, USA), and anti-FRS2α antibody (Santa Cruz Biotechnology). .. Membranes were then incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies, followed by signal detection using the enhanced chemiluminescence system (GE Healthcare, Buckinghamshire, UK).

Immunofluorescence:

Article Title: Evaluation of 89 Zr-Labeled Human Anti-CD147 Monoclonal Antibody as a Positron Emission Tomography Probe in a Mouse Model of Pancreatic Cancer
Article Snippet: The intensity of each band was quantified using ImageJ software (National Institute of Mental Health, Bethesda, MD, USA). .. For immunofluorescence staining, cells were grown on glass coverslips and fixed in cold methanol for 5 min. Nonspecific binding of the antibody was blocked by applying Block Ace reagent (Dainippon Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Article Title: Preclinical evaluation of 89Zr-labeled human antitransferrin receptor monoclonal antibody as a PET probe using a pancreatic cancer mouse model
Article Snippet: Objective Pancreatic cancer is aggressive and its prognosis remains poor; thus, effective therapy is urgently needed.. Transferrin receptor (TfR) is highly expressed in pancreatic cancer and is considered to be a good candidate for molecular-targeted therapy.. We radiolabeled and evaluated fully human anti-TfR monoclonal antibodies as a new PET probe for evaluating the biodistribution of the anti-TfR antibody in pancreatic cancer.

Article Title: C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model.
Article Snippet: Introduction: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor arising from the gastrointestinal tract and highly expresses mutated c-kit.. We aimed to develop a specific and sensitive method for detecting GISTs using radiolabeled anti-c-kit monoclonal antibody.. Methods: A mutated c-kit-expressing cell clone was established by transfecting an expressing vector of mutated c-kit gene into HEK293 human embryonic kidney cells.

Staining:

Article Title: Evaluation of 89 Zr-Labeled Human Anti-CD147 Monoclonal Antibody as a Positron Emission Tomography Probe in a Mouse Model of Pancreatic Cancer
Article Snippet: The intensity of each band was quantified using ImageJ software (National Institute of Mental Health, Bethesda, MD, USA). .. For immunofluorescence staining, cells were grown on glass coverslips and fixed in cold methanol for 5 min. Nonspecific binding of the antibody was blocked by applying Block Ace reagent (Dainippon Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Article Title: Preclinical evaluation of 89Zr-labeled human antitransferrin receptor monoclonal antibody as a PET probe using a pancreatic cancer mouse model
Article Snippet: Objective Pancreatic cancer is aggressive and its prognosis remains poor; thus, effective therapy is urgently needed.. Transferrin receptor (TfR) is highly expressed in pancreatic cancer and is considered to be a good candidate for molecular-targeted therapy.. We radiolabeled and evaluated fully human anti-TfR monoclonal antibodies as a new PET probe for evaluating the biodistribution of the anti-TfR antibody in pancreatic cancer.

Article Title: C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model.
Article Snippet: Introduction: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor arising from the gastrointestinal tract and highly expresses mutated c-kit.. We aimed to develop a specific and sensitive method for detecting GISTs using radiolabeled anti-c-kit monoclonal antibody.. Methods: A mutated c-kit-expressing cell clone was established by transfecting an expressing vector of mutated c-kit gene into HEK293 human embryonic kidney cells.

Glass:

Article Title: Evaluation of 89 Zr-Labeled Human Anti-CD147 Monoclonal Antibody as a Positron Emission Tomography Probe in a Mouse Model of Pancreatic Cancer
Article Snippet: The intensity of each band was quantified using ImageJ software (National Institute of Mental Health, Bethesda, MD, USA). .. For immunofluorescence staining, cells were grown on glass coverslips and fixed in cold methanol for 5 min. Nonspecific binding of the antibody was blocked by applying Block Ace reagent (Dainippon Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Nonspecific Binding:

Article Title: Evaluation of 89 Zr-Labeled Human Anti-CD147 Monoclonal Antibody as a Positron Emission Tomography Probe in a Mouse Model of Pancreatic Cancer
Article Snippet: The intensity of each band was quantified using ImageJ software (National Institute of Mental Health, Bethesda, MD, USA). .. For immunofluorescence staining, cells were grown on glass coverslips and fixed in cold methanol for 5 min. Nonspecific binding of the antibody was blocked by applying Block Ace reagent (Dainippon Pharmaceutical, Osaka, Japan) with 10% goat serum for 30 min. ..

Cell Culture:

Article Title: Preclinical evaluation of 89Zr-labeled human antitransferrin receptor monoclonal antibody as a PET probe using a pancreatic cancer mouse model
Article Snippet: Objective Pancreatic cancer is aggressive and its prognosis remains poor; thus, effective therapy is urgently needed.. Transferrin receptor (TfR) is highly expressed in pancreatic cancer and is considered to be a good candidate for molecular-targeted therapy.. We radiolabeled and evaluated fully human anti-TfR monoclonal antibodies as a new PET probe for evaluating the biodistribution of the anti-TfR antibody in pancreatic cancer.

Incubation:

Article Title: Growth-related skeletal changes and alterations in phosphate metabolism.
Article Snippet: Serum inorganic phosphate (Pi) levels are higher in children than in adults; however, the underlying mechanisms remain unclear.. Therefore, we herein attempted to elucidate the mechanisms altering Pi metabolism from youth to adulthood using 4-week-old (young) and 12-week-old (adult) mice.. Despite higher serum Pi levels, serum fibroblast growth factor 23 (FGF23) levels were lower in young mice, and the amount of FGF23 in bone tended to increase from youth to adulthood.

Article Title: Complex intrinsic abnormalities in osteoblast lineage cells of X-linked hypophosphatemia: Analysis of human iPS cell models generated by CRISPR/Cas9-mediated gene ablation.
Article Snippet: Equal amounts of protein were separated on SDS-PAGE and were transferred to PVDF membranes (BioRad Laboratories, Hercules, CA). .. After blocking with Blocking One P reagent (Nacalai Tesque Inc.) or Block Ace reagent (Dainippon Pharmaceuticals, Osaka, Japan), the membranes were incubated at 4 ◦C overnight with the following primary antibodies: antiDMP1 rabbit polyclonal antibody (TaKaRa, Shiga, Japan), anti-OPN rabbit polyclonal antibody (ProteinTech, Rosemont, IL, USA), antiPiT1 (SLC20A1) rabbit polyclonal antibody (H-130; Santa Cruz Biotechnology, Santa Cruz, CA), anti-SLC20A2 (PiT-2) rabbit polyclonal antibody (ProteinTech), anti-GAPDH goat polyclonal antibody (V-18; Santa Cruz Biotechnology), anti-phosphorylated ERK1/2 antibody, antiERK1/2 antibody, anti-phosphorylated CREB (Ser133) antibody, antiCREB antibody, anti-phosphorylated FRS2α antibody (Cell Signaling Technology, Beverly, MA, USA), and anti-FRS2α antibody (Santa Cruz Biotechnology). .. Membranes were then incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies, followed by signal detection using the enhanced chemiluminescence system (GE Healthcare, Buckinghamshire, UK).



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